Fig 1: Detection of TKTL1 expression on protein levels. The cell morphology in cell culture is shown in the left column (light microscope, phase contrast, magnification x40 plus digital magnification). The immunohistochemistry was performed with the two anti-TKTL1 antibodies JFC12T10 and the polyclonal prestige antibody; brown: DAB, counterstain: hematoxiline (blue), magnification x400. Western blot analyses were performed with JFC12T10 (J), USB antibody 1C10 (U) and the SigmaPrestige antibody (P). Marker (M) with molecular weights in kDa. β-actin is shown as loading control.
Fig 2: Effect of radiation on cell viability. (A) Representative dose–response curves of selected cell lines in response to radiation with doses between 2 Gy and 8 Gy. (B) Cell lines are arranged according to decreasing cell viability after radiation with 2 Gy. This dose lead to survival of a small number (2 ± 1%) of radiated HUVEC control cells in contrast to other tested doses where no HUVEC cells survived (not shown). Cell lines found positive for tktl1 mRNA are highlighted.
Fig 3: Effects of paclitaxel and cisplatin on cell viability. Representative dose–response curves of selected cell lines in response to paclitaxel (A) and cisplatin (B). Paclitaxel was used in concentrations ranging between 0.2 and 31.8 nmol/ml, and cisplatin was used in concentrations ranging between 0.1 and 25.4 nmol/ml. Cell lines are arranged according to increasing IC50 values, from sensitive to resistant cell lines, for paclitaxel (C) and cisplatin (D). Cell lines found positive for tktl1 mRNA are highlighted.
Supplier Page from MilliporeSigma for Anti-TKTL1 antibody produced in rabbit